Various assays are available to explore the molecular mechanism of anti-proliferatory samples. Anti-proliferation analysis will provide information on the mechanism of cell death induced by a sample and suggest anti-cancer potential. Mechanism based assays include:
• Cell cycle analysis
• Apoptosis (caspase activation, annexin V binding, sub-G1 DNA content, mitochondrial depolarisation)
• Senescence (SA-β-GAL activity)
• Autophagy (acidic vacuole quantification)
Numerous cell lines to choose from including: A549, B16F10, MeWo, HT-29, Caco-2. MCF-7, HeLa, PC3, Mia-PaCa, HepG2, U937 and Jurkat.