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Assay(s) of Interest:

(Choose all assays of interest)

Hoechst 33342/PI dual staining
MTT
Cell cycle analysis
Caspase 3 activation
Caspase 8 activation
Mitochondrial depolarisation using TMRE
Annexin-V/PI for apoptosis/necrosis
SA-β-GAL activity for senescence
Acidic vacuole quantification for autophagy
Inhibition of carbohydrate digestion using α-glucosidase inhibition
Inhibition of carbohydrate digestion using α-amylase inhibition
Glucose utilisation
DPPiv inhibition
Protein glycation inhibition
Anti-inflammatory activity using nitric oxide (NO) production in RAW 264.7 macrophages as an indication
β-cell function
Adipogenesis using 3T3-L1 differentiation as a target
DPPH scavenging activity
FRAP assay
ORAC assay
CAPe
Nitric oxide (NO) scavenging using Greiss method
Nitric oxide (NO) scavenging using DAF staining
Oxidative stress determination using CellRox
CYP3A4 inhibtion
Carboxylesterase inhibition
β-glucuronidase inhibition
Collagenase inhibition
Nitric oxide (NO) scavenging using Greiss reagent (targeting inflammation)
NF-ĸB nuclear translocation using immunocytochemistry (targeting inflammation)
COX-2 expression using immunocytochemistry (targeting inflammation)
iNOS expression using immunocytochemistry (targeting inflammation)
Phagocytosis using pHrodo Green E. coli BioParticles Conjugate (targeting inflammation)
Oxidative stress using CellRox (targeting fibroblast function)
Oxidative stress induced cell death using a staining protocol of Hoechst 33342, PI and Annexin V-FITC
Nitric oxide (NO) scavenging using Greiss method
Nitric oxide (NO) scavenging using DAF staining
NF-ĸB nuclear translocation using immunocytochemistry (targeting inflammation)
COX-2 expression using immunocytochemistry (targeting inflammation)
iNOS expression using immunocytochemistry (targeting inflammation)
Phagocytosis using pHrodo Green E. coli BioParticles conjugate
Hoechst 33342/PI dual staining
Measurement of reactive oxygen species (ROS) using CellRox
MitoTracker Green and TMRE staining for mitochondrial dysfunction
LipidTox Red or Green staining for steatosis
LipidTox Red or Green staining for phospholipidosis
LysoTracker Red staining for lysosomal dysfunction/phospholipidosis
Microbroth dilution susceptibility method (INT)
Microplate Alamar Blue assay (MABA)
Crude Extract
Fraction
Pure Compund
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